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In Vivo Assessment of Diabetes

SRI currently offers in vivo models for Type I and Type II Diabetes, including Diabetic Neuropathy Testing. 

Type I Diabetes models include:

• Non-obese diabetic mice (NOD) and related strains, model of spontaneous Type 1 diabetes (T1D) autoimmune disease. 
• Multiple Dose Streptozotocin (MD-STZ) Induced Diabetes (mice and rats)  

Type II Diabetes models include:

• Streptozotocin (STZ) Induced Diabetes (mice and rats)
• Alloxan Induced Diabetes (mice and rats)

Blood Glucose Testing

We perform various types of glucose tests:

• oral glucose tolerance test (OGTT)
• post prandial (rodent chow meal test)
• intraperitoneal glucose tolerance test (IPGTT)
• intravenous glucose tolerance test (IVGTT)          

Blood glucose can be measured on a fasting basis (collected after an 8 to 10 hour fast), randomly (anytime).  Blood glucose is monitored with a regular glucose meter or Analox Instrument for more accurate readings or for glucose levels >600 mg/dL-900 mg/dL (5 µl sample)).

Diabetes Rescue Model (mice or rats)

• Transplantation of pancreatic islets into the kidney capsule of diabetic animals.
• Nephrectomy—removal of the graft should cause hyperglycaemia— to reverse diabetes            

Streptozotocin (STZ) Induced Diabetic Neuropathy* (rats)

* The von Frey Monofilament Test is used to measure mechanical allodynia. 

Other related models:

• Continuous Monitoring of Drinking Water or other liquids using an automated cage system. The system can record the drinking pattern (24/7 undisturbed) in normal and/or diabetic rats.
• Micturition (urine production or metabolites) monitoring using an automated cage system. 
• Microsurgical Implant and Characterization of Medical Device Prototypes (e.g., glucose sensor testing and other components).
• Insulin pellets and device implants for slow release of insulin and/or insulin like compound delivery.

  Compound delivery include:  Intrathecal catheter (Yaksh's method), kidney capsule, intravenous, oral, subcutaneous, intradermal, intranasal, patch implant, Alzet and/or external pump placement.  Please ask if you need a custom design method of delivery or specific microsurgery.

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       Last Updated Jun 10, 2010